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List of phenotypic characters for signature "Piano_EE"

1. No developing embryos seen 48 hours after dsRNA injection
Injected worms stop producing embryos 48 hours after dsRNA injection and contain no live/developing embryos at this time.
Wild Type RNAi Phenotype
Not available Not available
2. Reduced fecundity of injected worm
Injected animals lay less than 100 embryos during their lifetime.
Wild Type RNAi Phenotype
Not available Not available
3. Osmotically/ pressure sensitive
Embryos rupture when placed on a 2% agar pad and covered with a coverslip.
Wild Type RNAi Phenotype
Not available Not available
4. Small embryos
Embryos are produced that are less than 50% of the normal size of wild-type embryos.
Wild Type RNAi Phenotype
Wild Type Wild Type
5. Catastrophic one-cell arrest
Embryos fail to divide and arrest as one-cell embryos.
Wild Type RNAi Phenotype
Wild Type Wild Type
6. Large cytoplasmic granules
Abnormally large lipid granules are observed in the cytoplasm of P0.
Wild Type RNAi Phenotype
Wild Type Wild Type
7. Excessive blebbing
Excessive shaking and movements are seen in the cell membrane or cytoplasm of one-cell or two-cell embryos.
Wild Type RNAi Phenotype
Not available Not available
8. Ectopic cleavage furrows
Extra cleavage furrows are seen in one or all cells of a one-cell to four-cell cell embryo.
Wild Type RNAi Phenotype
Wild Type Wild Type
9. Multiple cavities
Multiple vesicles, vacuoles, or cavities are seen during early embryogenesis.
Wild Type RNAi Phenotype
Wild Type Not available
10. Slow cell cycle
Embryos take longer to divide during the first and second division.
Wild Type RNAi Phenotype
Not available Not available
11. Abnormal one-cell shape
One-cell embryos have an altered shape.
Wild Type RNAi Phenotype
Wild Type Wild Type
12. Pseudocleavage absent
No pseudocleavage is observed before or during pronuclear migration.
Wild Type RNAi Phenotype
Wild Type Not available
13. Exaggerated pseudocleavage
Embryos have a much more pronounced pseudocleavage than normal.
Wild Type RNAi Phenotype
Wild Type Wild Type
14. Delayed at pronuclear contact
Embryos are either delayed or arrested at pronuclear contact.
Wild Type RNAi Phenotype
Wild Type Not available
15. Polar bodies abnormal
Aberrant number (fewer or more than two) or size (matches or exceeds size of early PNs) or internalization of polar bodes
Wild Type RNAi Phenotype
Wild Type Wild Type
16. Polar body reabsorbed
A polar body is absorbed in either the one-cell or two-cell embryo.
Wild Type RNAi Phenotype
Wild Type Wild Type
17. Indistinct maternal pronucleus
Maternal pronucleus has a blurry appearance. The nuclear envelope does not have a crisp circular shape.
Wild Type RNAi Phenotype
Wild Type Wild Type
18. Multiple maternal pronuclei
One-cell embryos have two or more maternal pronuclei.
Wild Type RNAi Phenotype
Wild Type Wild Type
19. Multiple paternal pronuclei or aberrant centrosomes
More than one paternal pronucleus is present in the one-cell embryo or there is a defect in centrosome structure.
Wild Type RNAi Phenotype
Wild Type Wild Type
20. Abnormal size of pronucleus
Maternal or paternal pronucleus is either too small or too large.
Wild Type RNAi Phenotype
Wild Type Wild Type
21. Failure to form pronucleus
Either the maternal or paternal pronucleus is absent.
Wild Type RNAi Phenotype
Not available Not available
22. Paternal pronucleus migrates
Instead of the maternal pronucleus migrating to the posterior end of the one-cell embryo, the paternal pronucleus migrates to meet the maternal pronucleus in the anterior end.
Wild Type RNAi Phenotype
Wild Type Not available
23. Pronuclear breakdown asynchronous
Instead of breaking down synchronously, the two pronuclei break down asynchronously in the one-cell embryo.
Wild Type RNAi Phenotype
Wild Type Wild Type
24. Pronuclei fail to migrate
Neither of the pronuclei migrate so they never meet.
Wild Type RNAi Phenotype
Wild Type Wild Type
25. Pronuclei meet more centrally
The maternal and paternal pronuclei meet more centrally instead of meeting in the posterior end of the embryo.
Wild Type RNAi Phenotype
Wild Type Wild Type
26. P0 spindle positioning defect
Altered P0 spindle placement causes either a symmetric first division, a division in which P1 is larger than AB, or a division in which the asymmetry is exaggerated such that AB is much larger than normal.
Wild Type RNAi Phenotype
Wild Type Wild Type
27. P0 spindle absent
No mitotic spindle is seen in P0.
Wild Type RNAi Phenotype
Not available Not available
28. P0 spindle does not rotate
P0 spindle fails to rotate and extends perpendicular to the long axis of the embryo.
Wild Type RNAi Phenotype
Wild Type Wild Type
29. Delayed P0 spindle rotation
P0 spindle rotates late.
Wild Type RNAi Phenotype
Wild Type Not available
30. Loose mitotic furrow
Once formed, the mitotic furrow can "slide" laterally.
Wild Type RNAi Phenotype
Wild Type Not available
31. Cytokinesis defect
Cells of the embryo attempt to divide but fail to form two daughter cells.
Wild Type RNAi Phenotype
Wild Type Not available
32. Multiple nuclei in 1, 2, or 4-cell embryos
Embryos are seen that contain more than one nucleus per cell in either the one-cell, two-cell, or four-cell embryo.
Wild Type RNAi Phenotype
Wild Type Wild Type
33. Aberrant nuclear reassembly
Nuclear envelope does not reassemble properly.
Wild Type RNAi Phenotype
Wild Type Wild Type
34. Exaggerated asynchrony
Asynchrony of the second division is exaggerated and P1 divides more slowly than normal.
Wild Type RNAi Phenotype
Not available Wild Type
35. Reversed asynchrony
P1 divides before AB.
Wild Type RNAi Phenotype
Not available Not available
36. Synchronous second division
AB and P1 divide synchronously.
Wild Type RNAi Phenotype
Wild Type Wild Type
37. Aberrant AB or P1 spindle orientation
The orientation of the spindle is aberrant in either the AB or P1 cell.
Wild Type RNAi Phenotype
Wild Type Wild Type
38. Division axis of ABa or ABp aberrant
ABa or ABp divide in the wrong orientation.
Wild Type RNAi Phenotype
Not available Not available
39. P1 aster abnormal
The morphology of the P1 aster is abnormal.
Wild Type RNAi Phenotype
Wild Type Wild Type
40. AB aster resembles P1 aster
The morphology of the AB aster resembles that of the P1 aster.
Wild Type RNAi Phenotype
Wild Type Wild Type
41. Aberrant cell-cell contacts at the four-cell stage
ABa, ABp, EMS, or P1 contacts fewer of its sister cells than in wild-type embryos.
Wild Type RNAi Phenotype
Wild Type Wild Type
42. ABa, ABp and EMS divide synchronously
EMS divides at the same time that ABa and ABp divide.
Wild Type RNAi Phenotype
Not available Not available
43. EMS divides after and is smaller than P2
EMS divides after and is smaller than P2.
Wild Type RNAi Phenotype
Not available Not available
44. P2 and EMS divide synchronously
P2 and EMS divide synchronously.
Wild Type RNAi Phenotype
Not available Not available
45. Nuclei reform next to cell division remnant
After division of either one-cell or two-cell embryos, the nuclei reappear next to the cell division remnant.
Wild Type RNAi Phenotype
Wild Type Wild Type
46. EMS fails to extend anteriorly
EMS fails to extend anteriorly and "hug" ABa.
Wild Type RNAi Phenotype
Wild Type Not available
47. EMS extends too far anteriorly
EMS extends too far anteriorly at the four-cell stage.
Wild Type RNAi Phenotype
Wild Type Not available