Cenix:14-d10 | RNAi_reagent | Cenix:14-d10
|
| Method | RNAi
|
| WormBaseID | WBRNAi00041149
|
| Reference | WBPaper00025054
|
|
Experiment (3)
|
|
Inhibits
| Predicted_gene | C25A1.9
| Source_evidence | ePCR | 1215 | WS150 Sequence/Annotation Release |
| | Gene |
C25A1.9
| Source_evidence | ePCR | 1273 | WS150 Sequence/Annotation Release |
| | | | Map_status | Unique_ePCR | I | 10185782 | 10187471 |
| | Transcript | C25A1.9
| Source_evidence | ePCR | 1273 | WS150 Sequence/Annotation Release |
| | WB_Predicted_gene | C25A1.9
| Inferred_automatically | RNAi_primary |
| | WB_Gene | C25A1.9
| Inferred_automatically | RNAi_primary |
| | Gene_target |
C25A1.9
| RNAiDB | ePCR | ePCR_unique |
| | | | Wormbase | BLAST | BLAST_wormbase_primary |
| WB_Homol | Homol_homol | C25A1:RNAi
|
| WB_Uniquely_mapped |
| Supporting_data |
External_Movie
| 14-d10-1-d1-1.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-1-d1-1.mov |
| | | 14-d10-1-d2-2.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-1-d2-2.mov |
| | | 14-d10-1-d3-3.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-1-d3-3.mov |
| | | 14-d10-2-d1-1.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-2-d1-1.mov |
| | | 14-d10-2-d2-2.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-2-d2-2.mov |
| | | 14-d10-2-d3-3.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-2-d3-3.mov |
| | | 14-d10-2-d4-4.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-2-d4-4.mov |
| | | 14-d10-2-d5-5.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-2-d5-5.mov |
| | | 14-d10-3-d1-1.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-3-d1-1.mov |
| | | 14-d10-3-d2-2.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-3-d2-2.mov |
| | | 14-d10-3-d3-3.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-3-d3-3.mov |
| | | 14-d10-3-d4-4.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-3-d4-4.mov |
| | | 14-d10-3-d5-5.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-3-d5-5.mov |
| | | 14-d10-3-d6-6.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-3-d6-6.mov |
| | | 14-d10-4-F-1.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-4-F-1.mov |
| | | 14-d10-4-F-2.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-4-F-2.mov |
| | | 14-d10-4-F-3.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-4-F-3.mov |
| | | 14-d10-4-F-4.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-4-F-4.mov |
| | | 14-d10-4-F-5.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-4-F-5.mov |
| | | 14-d10-5-F-1.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-5-F-1.mov |
| | | 14-d10-5-F-2.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-5-F-2.mov |
| | | 14-d10-5-F-3.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-5-F-3.mov |
| | | 14-d10-5-F-4.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-5-F-4.mov |
| | | 14-d10-5-F-5.mov | http://worm-srv2.mpi-cbg.de/ceimages/14/14-d10-5-F-5.mov |
|
Remark
| early embryonic: Spindle Elongation/Integrity |
| | early embryonic: 3/5 embryos show spindle related defects, in one of the cases the spindle is hardly visible, in others centration fails or is inaccurate, rocking is missing. This leads to chromosome segregation defects in two of the embryos. |
| | 60-70% embryonic lethality in 2/3 tests, escapers arrest at larval stage or develop into adults with egg laying defect. |
| Delivery | Delivered_by | Injection |
|
Results
|
Phenotype
| Emb
|
| | | Spindle Elongation/Integrity abnormal
|
| |
Phenotype_summary
|
Ste
| Result | No |
| | | | Reason | None_reported |
| | |
Stp
| Result | No |
| | | | Reason | None_reported |
| | | Emb | Result | Yes |
| | | EmbPheno | Result | Yes |
| | |
PostembLethal
| Result | No |
| | | | Reason | None_reported |
| | |
PostembPheno
| Result | No |
| | | | Reason | None_reported |
| | | Movies_images | Result | Yes |
| DB_info | Database | Phenobank2
| Gene&RNAID
| GeneID=947&RNAID=1077
|