This study reports the identification and phenotypic characterization of 661 genes with phenotypes in the early embryo (up to the 2-cell stage) in an RNAi survey of over 95% of C. elegans genes. In RNAiDB, experiment names from this study begin with "Cenix:". A phenotypic signature of 45 characters was generated for each of the 661 genes. The 661 Cenix Early Embryonic phenotypic signatures can now be queried using PhenoBlast.
In this study RNAi was performed using ORFeome clones for over 1,000 genes with transcripts enriched in the ovary with no previously identified embryonic phenotypes. 155 new genes with roles in embryogenesis were identified, raising the total number of known embryonic essential genes to ~1,800. Notably, over two-thirds of the newly identified genes showed partial penetrance lethality, suggesting that most genes with strong embryonic phenotypes have already been identified. Experiment names from this study begin with "PF:ORC_" in RNAiDB.
An article describing RNAiDB and PhenoBlast has been published in NAR: Gunsalus et al., 2004.
This study reports the first large-scale RNAi screen in C. elegans. Clones from an ovary cDNA library were screened for embryonic lethality and 81 genes with phenotypes in the early embryo were analyzed and grouped into phenotypic classes. Experiment names from this study begin with "KK:SP" in RNAiDB.
Development of RNAiDB is supported by grants from the National Science Foundation and the National Institutes of Health.