RNAi: Cenix:221-e5
Cenix:221-e5 | RNAi_reagent | Cenix:221-e5 | |||||
---|---|---|---|---|---|---|---|
Method | RNAi | ||||||
WormBaseID | WBRNAi00038683 | ||||||
Reference | WBPaper00025054 | ||||||
Experiment | Laboratory | TH | |||||
Author | Sonnichsen B | ||||||
Koski LB | |||||||
Walsh A | |||||||
Marschall P | |||||||
Neumann B | |||||||
Brehm M | |||||||
Alleaume AM | |||||||
Artelt J | |||||||
Bettencourt P | |||||||
Cassin E | |||||||
Hewitson M | |||||||
Holz C | |||||||
Khan M | |||||||
Lazik S | |||||||
Martin C | |||||||
Nitzsche B | |||||||
Ruer M | |||||||
Stamford J | |||||||
Winzi M | |||||||
Heinkel R | |||||||
Roder M | |||||||
Finell J | |||||||
Hantsch H | |||||||
Jones SJ | |||||||
Jones M | |||||||
Piano F | |||||||
Gunsalus KC | |||||||
Oegema K | |||||||
Gonczy P | |||||||
Coulson A | |||||||
Hyman AA | |||||||
Echeverri CJ | |||||||
Date | 24 Mar 2005 00:00:00 | ||||||
Inhibits | Predicted_gene | B0035.8 | Source_evidence | ePCR | 118 | WS150 Sequence/Annotation Release | |
F54E12.4 | Source_evidence | ePCR | 118 | WS150 Sequence/Annotation Release | |||
H02I12.6 | Source_evidence | ePCR | 118 | WS150 Sequence/Annotation Release | |||
Gene (3) | |||||||
Transcript | B0035.8 | Source_evidence | ePCR | 118 | WS150 Sequence/Annotation Release | ||
F54E12.4 | Source_evidence | ePCR | 118 | WS150 Sequence/Annotation Release | |||
H02I12.6 | Source_evidence | ePCR | 118 | WS150 Sequence/Annotation Release | |||
WB_Predicted_gene | B0035.8 | Inferred_automatically | RNAi_primary | ||||
F54E12.4 | Inferred_automatically | RNAi_primary | |||||
F55G1.3 | Inferred_automatically | RNAi_primary | |||||
H02I12.6 | Inferred_automatically | RNAi_primary | |||||
WB_Gene (4) | |||||||
Gene_target | B0035.8 | RNAiDB | ePCR | ePCR_best | |||
Wormbase | BLAST | BLAST_wormbase_primary | |||||
F54E12.4 | RNAiDB | ePCR | ePCR_best | ||||
Wormbase | BLAST | BLAST_wormbase_primary | |||||
H02I12.6 (2) | |||||||
F55G1.3 | Wormbase | BLAST | BLAST_wormbase_primary | ||||
WB_Homol | Homol_homol (4) | ||||||
Supporting_data | External_Movie (8) | ||||||
Remark | early embryonic: Sister Chromatid Separation (Cross-eyed) | ||||||
early embryonic: Highly reproducible chromatin segregation defect resulting in the typical cross-eyed nuclear separation pattern with tear shaped nuclei that stay attached to the newly forming cortex. Interfering chromatin bridges can result in failure of cytokinesis completion. Phenotype is more pronounced at four cell stage. | |||||||
90-100% of the progeny die during embryonic development. | |||||||
Delivery | Delivered_by | Injection | |||||
Results | Phenotype | Emb | |||||
Sister Chromatid Separation abnormal (Cross-eyed) | |||||||
Phenotype_summary | Ste | Result | No | ||||
Reason | None_reported | ||||||
Stp | Result | No | |||||
Reason | None_reported | ||||||
Emb | Result | Yes | |||||
EmbPheno | Result | Yes | |||||
PostembLethal | Result | No | |||||
Reason | None_reported | ||||||
PostembPheno | Result | No | |||||
Reason | None_reported | ||||||
Movies_images | Result | Yes | |||||
DB_info | Database | Phenobank2 | Gene&RNAID | GeneID=502287&RNAID=3505983 | |||
GeneID=509520&RNAID=3505983 | |||||||
GeneID=510211&RNAID=3505983 |