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RNAi: PF:GL1_6E4   

PF:GL1_6E4RNAi_reagentsjj_K08E7.3
MethodRNAi
WormBaseIDWBRNAi00008052
Gene_setGL1
ReferenceWBPaper00005599
Experiment LaboratoryKK
Date19 Nov 2002 00:00:00
Inhibits Predicted_geneK08E7.3 Source_evidenceePCR1054WS150 Sequence/Annotation Release
Gene K08E7.3 Source_evidenceePCR1054WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIV1256782812568978
TranscriptK08E7.3 Source_evidenceePCR1054WS150 Sequence/Annotation Release
WB_Predicted_geneK08E7.3 Inferred_automaticallyRNAi_primary
WB_Gene F55H2.4 Inferred_automaticallyRNAi_secondary
K08E7.3 Inferred_automaticallyRNAi_primary
WB_PseudogeneF55H2.4 Inferred_automaticallyRNAi_secondary
Gene_target K08E7.3 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
F55H2.4 WormbaseBLASTBLAST_wormbase_secondary
WBBLAST_wormbase_secondary
WB_Homol Homol_homol F55H2:RNAi
K08E7:RNAi
Supporting_data Movie (4)
RemarkPCR product contains flanking T7 promoter sequences
DeliveryDelivered_byInjection
Results Phenotype Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Delayed P0 spindle rotation
Division axis of ABa or ABp aberrant
Emb (2)
Nuclei reform next to cell division remnant
P0 spindle does not rotate
P0 spindle positioning defect
P1 aster abnormal
P2 and EMS divide synchronously
Stp RemarkObserved in >=10% of progeny.
Phenotypic_signature Discrete_pheno_char 1No
2No
3No
4No
5No
6No
7No
8No
9No
10No
11No
12No
13No
14No
15No
16No
17No
18No
19No
20No
21No
22No
23No
24No
25No
26Yes
27No
28Yes
29Yes
30No
31No
32No
33No
34No
35No
36No
37Yes
38Yes
39Yes
40No
41Yes
42No
43No
44Yes
45Yes
46No
47No
Phenotype_summary (7)