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RNAi: TH:300c6   

TH:300c6RNAi_reagentTH:300c6
MethodRNAi
WormBaseIDWBRNAi00002431
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Gene C36E8.5 Source_evidenceePCR1511WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIII40158934017836
Transcript C36E8.5.1 Source_evidenceePCR1511WS150 Sequence/Annotation Release
C36E8.5.2 Source_evidenceePCR1437WS150 Sequence/Annotation Release
C36E8.5.3 Source_evidenceePCR1358WS150 Sequence/Annotation Release
WB_Predicted_geneC36E8.5 Inferred_automaticallyRNAi_primary
WB_GeneC36E8.5 Inferred_automaticallyRNAi_primary
Gene_target C36E8.5 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
C36E8.5.1 RNAiDBePCR
C36E8.5.2 RNAiDBePCR
C36E8.5.3 RNAiDBePCR
WB_HomolHomol_homolC36E8:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA (C36E8.5-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for C36E8.5 function.
DIC phenotype -- Lack of male pronuclear migration; female pronucleus is located initially towards center of embryo or next to male pronucleus; sometimes 2 female pronuclei; female pronuclei drift towards male pronucleus; no bipolar spindle assembly.
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)