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RNAi: TH:340h12   

TH:340h12RNAi_reagentTH:340h12
MethodRNAi
WormBaseIDWBRNAi00002744
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneC05D11.3 Source_evidenceePCR424WS150 Sequence/Annotation Release
Gene C05D11.3 Source_evidenceePCR424WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIII64321216432587
TranscriptC05D11.3 Source_evidenceePCR424WS150 Sequence/Annotation Release
WB_Predicted_geneC05D11.3 Inferred_automaticallyRNAi_primary
WB_GeneC05D11.3 Inferred_automaticallyRNAi_primary
Gene_target C05D11.3 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolC05D11:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA (C05D11.3-RNA2; similar DIC phenotype, but progeny test was not embryonic lethal).
DIC phenotype -- Lack of male pronuclear migration (female pronuclear migration does not appear affected); proper bipolar spindle does not set up in most embryos. Some embryos have multiple pronuclei.
All embryos dead
Results PhenotypeEmb
Phenotype_summary (7)