Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 

RNAi: TH:340h5   

TH:340h5RNAi_reagentTH:340h5
MethodRNAi
WormBaseIDWBRNAi00002746
ReferenceWBPaper00004403
Experiment LaboratoryTH
Author (21)
Date16 Nov 2000 00:00:00
Inhibits Predicted_geneK06H7.1 Source_evidenceePCR319WS150 Sequence/Annotation Release
Gene K06H7.1 Source_evidenceePCR319WS150 Sequence/Annotation Release
Map_statusUnique_ePCRIII80996638100026
TranscriptK06H7.1 Source_evidenceePCR319WS150 Sequence/Annotation Release
WB_Predicted_geneK06H7.1 Inferred_automaticallyRNAi_primary
WB_GeneK06H7.1 Inferred_automaticallyRNAi_primary
Gene_target K06H7.1 RNAiDBePCRePCR_unique
WormbaseBLASTBLAST_wormbase_primary
WB_HomolHomol_homolK06H7:RNAi
WB_Uniquely_mapped
Remark Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction.
DIC phenotype -- Complex phenotype. In most embryos (e.g. movie1), eggshell appears fragile as witnessed by random yolk granule movements movements; pronuclei appear present, but fragility of embryos prevents thorough phenotypic characterization; no progress to the two cell stage; in other embryos (e.g. movie 2), defect in spindle assembly; also, no visible cleavage furrow; often large cytoplast on anterior side
DIC phenotype comment -- See also phenotypic description in Chase et al. (2000).
All embryos dead
Results (2)