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RNAi: WBRNAi00038529   

SpeciesCaenorhabditis elegans
Experiment Author Aoki J
Arai H
Inoue K
Inoue T
Sugimoto A
Suzuki Y
Tsujimoto M
Yamamoto M
Date07 Sep 2004 00:00:00
Genotypepaf-1(tj11); qaIs3102 (hsp::paf-2 RNAi)
TreatmentAnimals were allowed to lay eggs for 2-3 h and 4-5 h later the eggs were heat-shocked at 33 C for 20-25 min. Unhatched eggs were examined 16-18 h after being laid, and hatched but arrested L1 larvae were examined 48 h after being laid.
Inhibits (3)
RemarkMost embryos that were arrested during embryogenesis had a very lumpy morphology or were ruptured, with internal cells oozing through the holes in the epidermis. In some embryos, no MH27 staining was seen in the anterior part of the embryo, whereas the posterior part was enclosed by epidermal cells and elongated partially, which is indicative of anterior rupture during enclosure. Hatched but arrested paf-2 RNAi animals also exhibited morphology defects, including ectopic protuberances and a kinked tail.
Results Phenotype (2)
Phenotype_summary (7)